Basic Methods in Protein Purification and Analysis
| Series: | Industrial biotechnology |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Subject: | Proteins--Purification, Proteins--Analysis, Proteins--isolation & purification, Chromatography--methods, Electrophoresis--methods, Immunoprecipitation-- methods, Proteins--analysis |
| Authors: | Richard J. Simpson , Peter David Adams , Erica Golemis |
| Pages: | 436 pages |
| Binding: | Hardcover |
| ISBN: | 9780879698676 |
| Call No: | QP 551. B265 2008 |
Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.