Proteomics in Practice
| Series: | |
| Publisher: | Wiley |
| Subject: | Proteins--Analysis, Molecular biology, Proteome |
| Authors: | Reiner Westermeier , Tom Naven , Hans-Rudolf Höpker |
| Pages: | 502 pages |
| Binding: | Hardcover |
| ISBN: | 9783527319411 |
| Call No: | QP 551. P75673 2008 |
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.